To unlock the characters listed for Samurai 3, fulfill the corresponding requirement listed.

• Fuma Kotaro - Complete Orochi Chapter 1 (Battle of Odawara Castle)
• Gracia - Complete Wei Chapter 4 (Battle of Mt. Wu Xing)
• Maeda Toshiie - Complete Wei Chapter 4 (Battle of Ji Castle)
• Naoe Kanetsugu - Complete Samurai Chapter 3 (Battle of Hasedo)
• Nene - Complete Shu Chapter 4 (Battle of Mt. Wu Xing)
• Sasaki Kojiro - Complete Samurai Chapter 2 (Battle of Ji Province)
• Shibata Katsuie - Complete Wei Chapter 7 (Battle of Tong Gate)