Fulfill the following criteria to unlock the corresponding characters for Vs. Mode:

  • Casey Jones: Beat Stage 1 with Raphael
  • Evil Turtlebot: Beat Stage 3 with any turtle
  • Hun: Beat Stage 6 with Michaelangelo
  • Splinter: Beat Dojo Stage 3 with Leonardo
  • Shredder: Beat Shredder with any turtle
  • Oroku Saki: Beat Saki with any turtle
  • Yoshi Hamato: Beat Challenge Mode with any turtle