To unlock the following Challenge Games increased difficulties, complete the Challenge Game listed in the fashion listed.
- Banana Grab (Level 2) - Banana Grab (80+ Bananas)
- Banana Grab (Level 3) - Banana Grab (Level 2, 100+ Bananas)
- Banana Grab (Survival) - Banana Grab (Level 3, 140+ Bananas)
- Booster Battle (Level 2) - Booster Battle (defeat Funky Kong)
- Booster Battle (Level 3) - Booster Battle (Level 2, defeat Dixie Kong)
- Booster Battle (Survival) - Booster Battle (Level 3, defeat Diddy Kong)
- Orange Picker (Level 2) - Orange Picker (60+ Oranges)
- Orange Picker (Level 3) - Orange Picker (Level 2, 100+ Oranges)
- Orange Picker (Survival) - Orange Picker (Level 3, 130+ Oranges)
- Rolling Panic (Level 2) - Rolling Panic (100+ Points)
- Rolling Panic (Level 3) - Rolling Panic (Level 2, 150+ Points)
- Rolling Panic (Survival) - Rolling Panic (Level 3, 250+ Points)
- Speed Climb (Level 2) - Speed Climb (15 seconds or less)
- Speed Climb (Level 3) - Speed Climb (Level 2, 20 seconds or less)
- Speed Climb (Survival) - Speed Climb (Level 3, 28 seconds or less)
- Throw N Crush (Level 2) - Throw N Crush (40 seconds or less)
- Throw N Crush (Level 3) - Throw N Crush (Level 2, 50 seconds or less)
- Throw N Crush (Survival) - Throw N Crush (Level 3, 60 seconds or less)